ABSTRACT
OBJECTIVE@#To investigate the effect of basic fibroblast growth factor (FGF-2)on survivin and subcellular location of Smac in human small cell lung cancer (SCLC) cell NCI-H446.@*METHODS@#Western blot was used to detect the expression of survivin protein induced by FGF-2. The release of Smac from mitochondria to cytoplasm affected by FGF-2 was observed by Western blot and immunofluorescence. Apoptosis of NCI-H446 cells was detected with flow cytometry and Hoechst 33258 staining.@*RESULTS@#The expression of survivin could be up-regulated in response to FGF-2 treatment in NCI-H446 cells, and the level of survivin expression is related to the concentration and time of FGF-2 treatment. FGF-2 could inhibit the release of Smac from the mitochondria to cytoplasm induced by serum starving. FGF-2 could inhibit the apoptosis induced by serum starving.@*CONCLUSION@#FGF-2 up-regulates the expression of survivin protein in NCI-H446 cells, and blocks the release of Smac from mitochondria cytoplasm. Survivin and Smac might play important roles in the apoptosis inhibited by FGF-2.
Subject(s)
Humans , Apoptosis , Apoptosis Regulatory Proteins , Cytoplasm , Metabolism , Fibroblast Growth Factor 2 , Pharmacology , Inhibitor of Apoptosis Proteins , Intracellular Signaling Peptides and Proteins , Metabolism , Lung Neoplasms , Metabolism , Pathology , Microtubule-Associated Proteins , Mitochondria , Metabolism , Mitochondrial Proteins , Metabolism , Small Cell Lung Carcinoma , Metabolism , Pathology , Survivin , Tumor Cells, CulturedABSTRACT
OBJECTIVE@#To determine the expressions of Notch1, Jagged1 and vascular endothelial growth factor (VEGF) in human non-small cell lung cancer (NSCLC) and to explore its clinical pathological significance.@*METHODS@#Immunohistochemical SP method was used to detect the expressions of Notch1, Jagged1 and VEGF in 65 patients with NSCLC and 15 normal epithelial tissues of the lung, and the relationship between them and clinic-pathological parameters were analyzed.@*RESULTS@#The positive rates of Notch1, Jagged1 and VEGF in NSCLC were 81.5%, 83.1% and 93.8%, respectively, higher than those in normal epithelial tissues of the lung (P<0.05). The positive expression levels of Notch1 and VEGF were closely associated with the tumor stage and the lymph node metastasis (P<0.05). The positive expression levels of Jagged1 was positively correlated with the pathological type and lymph node metastasis. There was a positive correlation between Notch1, Jagged1 and VEGF.@*CONCLUSION@#Notch1, Jagged1 and VEGF protein may play an important role in the pathway of carcinogenesis and progression of NSCLC. The up-regulation of Notch1, Jagged1 and VEGF protein expression probably predict NSCLC carrying relatively strong permeation and metastasis.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Calcium-Binding Proteins , Metabolism , Carcinoma, Non-Small-Cell Lung , Metabolism , Pathology , Gene Expression Regulation, Neoplastic , Intercellular Signaling Peptides and Proteins , Metabolism , Jagged-1 Protein , Lung Neoplasms , Metabolism , Pathology , Lymphatic Metastasis , Membrane Proteins , Metabolism , Neoplasm Staging , Receptor, Notch1 , Metabolism , Serrate-Jagged Proteins , Vascular Endothelial Growth Factor A , MetabolismABSTRACT
<p><b>OBJECTIVE</b>Gingival crevicular fluid (GCF) analysis of various inflammatory mediators has been investigated as a means of providing objective criteria of peri-implant tissue health. In this report, the crevicular fluid levels of interleukin-8 (IL-8), GCF volume and clinical parameters were studied.</p><p><b>METHODS</b>GCF was sampled from 35 healthy and 35 inflamed sites of implantation patients. IL-8 levels were analysed using ABC-ELISA. Clinical parameters were measured, and data analysis was performed using the software package SPSS10.0.</p><p><b>RESULTS</b>Significant difference was observed between healthy implant sites and peri-implantitis sites. GCF volume was positively correlated with PD, PI, GI and MOB. The total amount of IL-8 was positively correlated with PD and GI.</p><p><b>CONCLUSION</b>This investigation suggested that GCF volume and IL-8 cytokine may be of value as a diagnostic and prognostic marker for peri-implantitis.</p>